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Promega
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DiscoverX corporation
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Active Motif
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Promega
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National Institute of Standards and Technology
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Image Search Results
Journal: bioRxiv
Article Title: Multiplex base editing to convert TAG into TAA codons in the human genome
doi: 10.1101/2021.07.13.452007
Figure Lengend Snippet: a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Article Snippet: All
Techniques: Modification, RNA Sequencing